Objective: The ideal nourishment for newborns with low birth-weight is Breast milk. The purpose of the study was to determine the effect of natural-Feeding education given to mothers of low-birth-weight infants, on the mothers’ Breast-Feeding self-efficacy level, Breast-Feeding success, and the growth of the infants.Methods: The study was conducted in a quasi-experimental way. The study group consisted of a total of 85 low-birth-weight infants and their mothers who had been treated in the neonatal clinics of two hospitals. The mothers included in the test group were given Breast-Feeding education for half an hour per day, during the first 5 days of their hospitalization. Home visits were carried out at the homes of the participants from both the test and control groups, until the infants reached 6 months of age. For collecting data, the following forms were used: Personal Information Form, Breast-Feeding Self-Efficacy Form, LATCH Breast-Feeding Assessment Tool, and Anthropometric Measurements Form. For analyzing the collected data, percentage, arithmetic mean, standard deviation, and chi-square we used, and, in independent groups, t-tests were employed.Findings: It was determined that natural-Feeding education given to the mothers increases their Breast-Feeding self-efficacy levels and success in Breast-Feeding (P<0.05). It was found that in the test group, the rate of Feeding the infants exclusively with Breast milk is higher in comparison with the control group (P<0.001).Conclusion: Results of the study indicate that natural-Feeding education increases Breast-Feeding self-efficacy levels, Breast-Feeding success, and the Breast-Feeding duration.